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FERMENTATIVE ACTIVITY OF SORGHUM PLANTS UNDER APPLICATION OF HERBICIDE AND PLANT GROWTH REGULATOR

Author(s) Karpenko V.P., Doctor of Agricultural Sciences, Professor, Vice-Rector for Research and Innovative Activity, Uman National University of Horticulture, Ukraine
Shutko S.S. , , , Uman National University of Horticulture
Category Plant physiology
year 2018 issue №2, 2018
pages 68-73 index UDK 633.174:581.1:631.348:631.811.98
DOI 10.31395/2310-0478-2018-21-68-72 (Link)
Abstract Sorghum is a relatively new grain crop, which active implementation into crop rotation is limited by a range of factors including a little-approbated technology of its growing. In connection with it, it is reasonable to substantiate separate elements of the technology of its growing based on physical-and-biochemical reactions of Sorghum plants under the influence of physiologically active substances (namely, herbicide and plant growth regulator). Sorghum plants during vegetation are effected by stress factors of different nature and active forms of oxygen (AFO) are formed in plant cells in response to them and these forms in turn can provoke increase in the activity of fermentsantioxidants. Thus such physiologic-and-biochemical reaction of plant organism can serve as one of the ways for revealing the depth of influence of chemical and biological preparations on physiologic-and-biochemical state of Sorghum plants. Results of the researches concerning the influence of physiologically active substances (herbicide and plant growth regulator) on passing of fermentative processes in Sorghum plants are presented in the article. The purpose of the article was to ascertain the influence of different norms of Peak 75 WG herbicide (10; 15; 20; 25 g/ha) under various methods of use of Regoplant plant growth regulator (treatment of sowing material (250 ml/t) and crops (50 ml/ha)) on the activity of individual ferments of oxidoreductase class. The objects of the research were Sorghum plants (Sorghum orysoidum) of Tytan variety, Peak 75 WG herbicide and Regoplant plant growth regulator. Laboratory analyzes were carried out in samples of leaves taken according to the development phases of Sorghum plants. The activity of the ferments of oxidoreductase class: catalase (KF 1.11.1.6), peroxidase (KF 1.11.1.7) and polyphenol oxidase (KF 1.10.3.1) was estimated according to the method described by Kh. M. Pochynok. Researchers have shown that application of Peak 75 WG herbicide in the norms of 15-20 g/ha in combination with Regoplant plant growth regulator (50 ml/ha - on crops + 250 ml/t - seed treatment) contributed to the greatest increase in the activity of individual ferments of oxidoreductase class. Under these conditions, the activity of certain ferments of oxidoreductase class in the study of the phase of Sorghum plants development was increased: by 22-49% for catalase, 18-39% for peroxidase and 45-53% for polyphenol oxidase. This may indicate both about activation of detoxification processes in Sorghum plants, and physiological-and-biochemical processes, which determines formation of crops productivity.
Key words herbicide, plant growth regulator, Sorghum, fermentative activity, catalase, peroxidase, polyphenol oxidase
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